I planning on using DREADDs with a conditioned place preference or 2 bottle preference test.
CPP test is short, about 20 minutes and the 2 bottle test would be an hour.
How long should I wait after giving the injection before starting the experiment?
This would depend on the pharmaokinetics of the agent. The time from adminstration to brain concentration, varies with route of adminstration and lipophillicity of the compound.
We typically wait 15-30mns post injection. CNO should normally be having effects for up to two hours after administration so you should be able to fit everything in.
Hi, in our hands a 0.3 mg/kg i.p. injection 30 minutes prior to the start of the behavior works well, and lasts up to six hours. We have used this for locomotor, operant and free drinking behaviors.
Please note that many studies have been conducted with doses that are very high, and likely pharmacologically active. You should do a simple experiment in sham or control virus animals with the CNO dose prior to conducting your experiment. I was surprised to see several labs routinely using 5 or even 18 mg/kg at SfN. There are some recent papers showing that higher doses are pharmacologically active in dopamine related behaviors.
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