Hello to everyone, I work with PBMC from healthy donors extracted by ficol. I do experiments where I measure the activity of PBMC - adcc activity - capacity of spheroid infiltration I have recently observed a change in the behaviour of PBMC from the same donor that I use, with a lower activity (10 fold less), they are about 10 months old. Their viability is still very good but the activity has dropped drastically. I am wondering if there is a recommended ideal storage time for PBMC? Thank you in advance, Have a nice day,

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