I've cell stained 30, 40, and 50 micron sections before but not thicker sections like 100 micron so just wondering if any of you might know the optimal incubation time in Cresyl-violet . I will of course trial test this but a lead would be great.
May I ask how long you stained the 40µm sections?
I´m trying 15 Min with 1% Solution. But results are nonsatisfying; tissue /or cells/ are not completely stained. did you stain also floating sections?
For my PhD dissertation: If Chapter 1 is a systematic literature review (QUANTS/QUALS) while Chapters 2, 3, and 4 are empirical chapters (QUALS), is this considered a mixed methods research...
16 June 2024 3,220 5 View
--
11 May 2024 9,741 0 View
I would like to better understand the influence of the morphological polymer characteristics on adhesive properties. What is the influence and the role of amorphous phase? and that of crystalline...
12 October 2023 1,486 5 View
How is AI redefining the future of Educational leadership and management?
14 May 2023 8,193 14 View
Dans le cadre de mon mastère spécialisé BIM à Ecole des Ponts ParisTech, je recherche des ouvrages ou des articles traitant de la gouvernance de la donnée et de la géométrie dans une maquette...
19 April 2023 3,023 2 View
Hello all, I'm trying to isolate and expand human primary renal fibroblasts from nephrectomy samples using an explantation technique. This technique has already been used successfully in my lab...
19 September 2021 4,766 5 View
Good afternoon everybody, I currently work on Liver Hepatocellular Carcinoma C3A cells. After Serum starving the cells, I would like to stimulate them with Serum media for 15 minutes and look at...
21 July 2020 5,374 0 View
Hello everyone, I am conducting a PLS research, I find Path estimates with p
17 July 2020 9,326 6 View
Can the position, grade, number of publication of scientists that authored a paper influence the visibility (acceptance, read, recommendation, citation,....) of an article during the submitting...
14 July 2020 3,221 8 View
Hi, how do you insert the conception of health (cultural, societal) in your Context approach ? Did you include collaboration and when ? For my internship i'm thinking about, it seems a...
21 November 2019 7,416 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View