Collin Gagne

3 Questions 1 Answers 0 Followers

Questions related from Collin Gagne

How long for vitamin D to be uptaken by Caco-2 cells and to reach saturation?

Doing an in vitro study with nanostructured lipid carriers with vitamin D and with just vitamin D alone, and just trying to figure out the optimal incubation time so I don't stop it too late...

24 February 2020 9,201 2 View

What antibody (for IBA1 or otherwise) works best to image microglia in 4% paraformaledhyde-fixed rat brain tissue?

I've been trying various iba1 antibodies with either Alexa 488 or 647 and am getting a lot of background and have never once seen anything close to resembling a microglia. I've tried blocking with...

19 June 2019 935 5 View

What antibody (for IBA1 or otherwise) works best to image microglia in 4% paraformaledhyde-fixed rat brain tissue?

I've been trying various iba1 antibodies with either Alexa 488 or 647 and am getting a lot of background and have never once seen anything close to resembling a microglia. I've tried blocking with...

06 June 2019 2,940 4 View