How long does non-perfused embryo heads take to submerge in 30% sucrose PBS?

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Chinaza Godswill Awuchi

Check https://www.nature.com/articles/nprot.2015.002?message-global=remove

Also check https://www.jefferson.edu/content/dam/skcc/Sample%20prep-frozen%20embedding.pdf

Samantha Cavalcante

Thanks for your help Chinaza! Your links were very helpful to me. Now I now that usually takes 48 hours or a little bit more to non-perfused embryo brains to sink.

Kiprotich Kiptum

Check this paper.

Article Clearing for Deep Tissue Imaging