How long does it take to revive Huh 7 Cells from liquid N2? What to do if the cells appear shiny but not displaying its morphology after 10days?

More Meyyammai Swaminathan's questions See All

What is the difference between coordination polymer, MOFs, MCOFs?

Wherever I have referred, it puts me in a dilemma about the exact differences between these. It would be really helpful if someone can put me out of this trouble.

21 July 2024 3,045 3 View

How to calculate Battery Pack Size for Li-ion Battery based on the Power requirement ?

Power Requirement : 100 Watts Voltage of Li - ion Battery Pack : 12V/24V

08 October 2023 9,092 0 View

Ow o calculate any PEM Fuel Cell Stack Size and select the correct Electrolyser based on the Power Requirement ?

Power Requirement is 100 Watts I am developing a prototype model of PEM Fuel Cell Electric Vehicle. So the max speed will be 1.4 m/s or approx. 5.00 kmph. For this I have calculated the power...

08 October 2023 2,281 0 View

Is Conocarpus tree a friend or Foe?

Reports are coming up that Conocarpus tree is harmful. It has both beneficial as well as harmful effects. Plant it cautiously. Please share your experiences and...

03 May 2023 1,352 1 View

Lumbar spine Finite element - How to model ligaments in ABAQUS using connectors?

Hello everyone, I have L4-L5 lumbar spine FE model in ABAQUS. I need some information regarding the connectors in ABAQUS to model the ABAQUS. Since connectors do not have a cross-section, how to...

19 April 2023 5,608 0 View

Lysis buffer without DTT for extracting cytoplasmic proteins?

Hi, I am trying to extract cytosol-enriched and nuclear-enriched fractions from cultured cells. For the cytoplasmic extraction, I am using a hypotonic lysis buffer that contains DTT...

13 March 2023 9,327 2 View

Why does C=S have a higher chemical shift in 13C NMR over C=O; given oxygen is more electronegative?

In 13C NMR, we see that C=S resonates around 170-180 whereas C=O at 150-160 ppm. Given oxygen is more electronegative; shouldn't it be vice-versa?

04 July 2022 8,057 5 View

Why is chorine considered as a ligand in coordination chemistry?

Given chlorine is an anionic ligand, there is a sharing of electrons between Cl and the metal ion (definition of covalent bonding). Can the bonding between be considered Co-ordinate? if so why?

01 July 2022 3,958 6 View

Is there a technique other than FIB for preparing a TEM sample from a wire of diameter 1 mm?

To prepare TEM samples from a wire of 1 mm in diameter, I am looking for a suitable preparation method other than the FIB.

14 January 2022 463 3 View

Why are metal complexes readily soluble in chlorinated solvents?

Normally, polarity affects the solubility of a metal complex. However, my complex has excellent solubility in DCM and chloroform but is sparingly soluble in acetonitrile which has a higher dipole...

15 December 2021 2,917 4 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How can we conduct DFT for solutions or mixtures ?

Can we conduct DFT studies for Ionic Liquids with Amines at various concentrations? If so, how can we proceed with these studies? Additionally, what parameters, besides IR, NMR, and HOMO-LUMO, can...

18 July 2024 319 1 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View

Which is true nano nitrogen or nano urea?

Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?

28 June 2024 2,885 6 View

Why does a nitrogen adsorption-desorption isotherm form two loops?

BET analysis

27 June 2024 6,964 1 View

Can a liquid metal-ligand sample be directly analyze in flame atomic absorption spectrosocopy?

Can I directly analyze a liquid metal-ligand sample without acid digestion in flame atomic absorption spectroscopy?

25 June 2024 1,866 2 View