Meyyammai Swaminathan

2 Questions 1 Answers 0 Followers

Questions related from Meyyammai Swaminathan

I want to revive 3T3 adipocytes from Liq Nitrogen. Can i store in DMEM+FBS+Penstrep+DMSO or need to maintain in differentiation medium before storing?

The papers that I read on adipocytes mainly discuss to culture in DMEM+FBS+Penstrep and transferred into MDI Differention media for treatment. I would like to check the viability of the cells...

28 January 2020 4,360 1 View

How long does it take to revive Huh 7 Cells from liquid N2? What to do if the cells appear shiny but not displaying its morphology after 10days?

These cells have been stored in liquid nitrogen for the last 5 months. I have tried reviving from 5 different Passages (which i have done succesful revival previously).

21 December 2018 1,842 2 View