[dithiothretol DTT sputasol + centrifugation].
What are the steps prior to culturing on agar plates for routine microbiology of cystic fibrosis patients?. How many centrifugation steps are used and at what speed in rpm.
I found a document but it is not time specific. I am curious to know how long it takes to see if this can be made more rapid as I have research concepts and journals to try new methodology.