The plasma was in a cryogenic tube with a volume of 500ul and previously stored in -80C freezer for months. I would like to know how long does it take to melt at room temperature? Does anyone have related experience to share? Thanks!
300microlitres is a very very small volume.
(0.3ml, right?)
It depends on how well-insulated that vessel is, and the conditions at room temperature.
(surrounded by 10cm of foamed insulation? Many tens of minutes)
(held in a thin-walled vessel surrounded by still air? A handful of minutes)
(held in a thin-walled vessel with a stream of dry air flowing over it? A minute or less)
etc.
James Garry Thanks James! I may not melt the sample in the room air, probably on the ice to slow down.
Mingming Zhang An excellent idea - Pyrex can probably tolerate a fast warm-up, plastics such as FEP, or PFA, or PTFE, might benefit from a gentler warm-up.
I'd probably just lift it from the freezer, and suspend it in air (somewhere safe) for 10min or so. You'll have a little frost on the outside, but if it's capped/sealed (Parafilm?) then all should be well.
- Badges
- Science topic
- Similar topics
- Mathematical Sciences
- Graphs