Hi, i´m currently transforming DH5a via electroporation using pGEM-T and a 1 kb ADH insert. I used heat-shock in my previous attempt and i observed only white colonies after approximately 24-36 hours at 30°C on X-gal/IPTG/Amp(200ul/mL) LB plates, though miniprep and digestion gels didn´t show any band close to my insert being released. I´ve read that DH5a usually takes a little longer to grow than wild-type as it it recA-, and that with a plasmid it may take a little longer. I left my electroporated cells at 37° overnight (16 h) this time, and i still don´t see colonies. Any advice would be really appreciated, thank you in advance.

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