What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

What is the difference between SEM and TEM and between FTIR and EDX in soil and plant?

If I want to know the changes (for example in plants or soils) as affected by specific substance on nano substance; when I use SEM or TEM and why? As well, when I use FTIR or EDX and why also? Is...

28 January 2021 5,070 9 View

Rmse in lstm when forecasting time series?

iam working on long memory time series data and i use lstm neural networ to train data and i want to understand how rmse is calculated is it calculated in each iteration separately or in each...

23 December 2020 6,740 4 View

Doping of r-GO?

if the percentage of doping Au into rGO is too small 1~10%, is it not enough to prevent restacking of r-GO in xrd results, rGO peak appeared at 22.2 and also the peaks of gold nanoparticles so...

21 December 2020 6,091 3 View

XRD peaks for gold nanoparticles ?

is the concentration of gold salt that were added will affect on the peaks appeared. i added a very little amount of gold salt to dope graphene oxide and not all the peaks that proved the...

17 December 2020 9,275 3 View

I saw sparkles in my samples?

i am trying to dope boron in graphene oxide , and after i take out my samples from the oven i put them in the microwave for 40 sec , and i saw sparkles . and fume also i couldn't understand why

16 November 2020 6,852 5 View

I am looking for efficient texture analysis software for MRI images analysis with free acess?

i am looking for efficient texture analysis software for MRI images analysis with free access?

09 October 2020 3,737 2 View

What is the alternative test that can be used instead of ANCOVA when the Homogeneity of regression slopes doesn't meet?

I have Pre test as a (covariate variable) and post test as a (dependent variable) and the treatment for the experimental group and the control group. and the assumption of Homogeneity of...

21 August 2020 7,196 3 View

What if find that the reviewer goes through your paper just to reject it?

What you should do if you found that one of the reviewers goes through your paper just to reject it and most of his comments are irrational and not constructive. Finally, he rejected the paper...

20 April 2020 5,336 51 View

Giemsa stain preparation tips, for chromosomes' staining, any advises?

Can any one advise me about the best protocol for Giemsa stain preparation? it gives me faint blue color not the ideal purple color, that makes the investigation difficult and the color is not...

16 March 2020 6,656 3 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View