I want to check the phosphorylation effect on ERK, does anyone know which protein should I use as positive control?

I want to check the phosphorylation effect on ERK, do anyone know which protein should I use as positive control?

01 February 2015 7,692 2 View

Can I culture Mono Mac 6 with DMEM?

Can I culture Mono Mac 6 with DMEM? Usually we culture mono mac 6 with RPMI, but I want to do some transmigartion experiment and do not want to change medium. Thanks

01 February 2015 9,491 2 View

ERK Phosphorylation problem in hek cells?

I made a stable transfected HEK 293 cell line to expressing my protein, and now I want to use the protein containing supernatant(FCS free) to stimulate the normal HEK 293 cell again, to see...

01 February 2015 411 3 View

Why do Raji cell become adherent after 24 hours serum starvation?

Raji cells were cultured in serum-free medium, they all adherent after 24 hours, does anyone know why is this the case?

01 February 2015 2,223 2 View

How can I elute the chitin bind protein from chitin beads?

Thanks in advance.

11 December 2014 6,074 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View