How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can I use human fibronectin with rat cells?

I'm trying to make a experiment in which i use fibronectin as a surface coat, but the cells are from Rat (C6 glioma and endothelial cells) and the fibronectin we have here is from human. Can I mix...

23 August 2020 8,503 4 View

Hi. I am doing a western blotting investigation, and found in different cell lines bands with different weights... Why this hapenned?

I am using anti-fibronectin antibodies in western blotting experiments, and in one breast cancer cell line, I found ~230kDa bands, but in other cell line of this cancer, I found bands of ~35kDa...

02 July 2020 10,015 3 View

Which statistic test to use to compare 2 samples in 6 conditions?

Hello, I am testing the adherence of cells in 2 different cultures (presence of BSA or presence of fibronectin). Each one of these cultures is tested in 5 different conditions (in the presence of...

29 April 2020 7,687 3 View

Transwell Tregs migration assay: fibronectin coating?

I performed this assay many times but without any consistent result. I usually isolate mouse spleen CD4+CD25+ cells, seed 100K non-activated cells on 3um-pore size membranes (24well plate,...

08 November 2019 3,030 1 View

Growth of dental pulp stem cells in glass coverslips?

I want to growth dental pulp stem cells in glass coverslips in order to induce differentiation into neurons. Months ago I used 5 ug/ml of poly-l-ornithine and 50 ug/ml of fibronectin as a matrix...

09 October 2019 7,502 1 View

Appropriate concentration of fibronectin to coat plates for HSCs culture?

I'm trying to culture hematopoietic stem cells of mice, referring to an article, in which fibronectin-coated plates are used. Since pre-coated plates are expensive, I'm thinking about coating...

28 August 2019 2,701 2 View

Appropriate concentration of fibronectin to coat plates for HSCs culture?

I'm trying to culture hematopoietic stem cells of mice, referring to an article, in which fibronectin-coated plates are used. Since pre-coated plates are expensive, I'm thinking about coating...

07 August 2019 7,827 2 View

Cells attach to plate but after 48 hours, they are floating?

I am trying to grow an immortalized cell line that has done well before, but now are no longer attaching. The cells attach fine post thaw, but over the course of 48-72 hours, all the cells...

06 July 2019 9,684 7 View

What is the process by which dishes are coated with Fibronectin?

What is the actual process that leads to fibronectin coating glass dishes? As far as I understand for collagen it works, by bringing the solution's ph to the isoelectric point to make it...

26 June 2019 3,540 2 View

Hello everyone, Can anyone tell me the optimum concentration of fibronectin to coat polystyrene plates to culture multiple myeloma cells (RPMI 8226)?

Currently, I am using RPMI 8226 and want to culture this cell on polystyrene substrate. I have to coat polystyrene substrate with fibronectin to ensure better cell attachment. I am wondering what...

05 June 2019 9,720 2 View