I am using PEGylated fibronectin to make hydrogels and I noticed some protocols say it can be stored at -20C until use but I was wondering how long it can be stored for?
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...
20 June 2024 393 0 View
Hi everyone, I am currently working with the LUHMES cell line from ATCC and have run into a few issues. First, the protocol for culturing them involves coating plates with 50 µg/mL...
01 April 2024 9,100 0 View
I am looking to get a placental cell line that Fibronectin FN1 endogenously expresses. My cell line choices are HTR-8/SVneo and JEG-3, but when I checked the FN1 expression in these cell lines...
03 January 2024 5,367 0 View
I need to enrich for mitotic cells for immunofluorescence. Since the mitotic cells are rounded I need to increase the adherence to the surface of the plate. So far I have been using only...
28 November 2023 9,614 0 View
Hi, I have extensive 2D and 3D spheroid cell culture experience and I am considering a multi-cellular culture whereby I layer cell types on top of each other, for example stromal fibroblasts...
23 October 2023 2,131 2 View
Hello! I am trying to make a monolayer using retinal endothelial cells. I followed the protocol I have for brain cells (which has worked for me in the past), but after adding FITC I found that the...
26 March 2023 3,281 0 View
I'm about to start some in vitro work using human brain microvascular endothelial cells (HBMECs). The company's (Innoprot) suggestion is these cells should be seeded in their optimised medium and...
06 March 2023 8,240 0 View
We are attempting to differentiate hiPSCs to cortical neurons and will be growing them on glass coverslips. We have to triple coat the glass with poly-L-ornithine (PLO), laminin and fibronectin....
13 February 2023 8,675 0 View
Is one superior to the other? Does it matter at all and will it have any impact the properties of the cells?
19 September 2022 4,420 0 View
Good evening, I have started my culture with HUVEC cells and HAoEC, I use Endothelial Cell Medium (Cat1001) from sciencell, so the complete medium contains FBS, antibiotics and endothelial cell...
09 July 2022 5,836 1 View