How long can i put my sciatic nerve tissue in the formalin solution for future immunofluroscent staining?

Xue Deng @Xue_Deng2

05 May 2018 0 406 Report

how long can i put my sciatic nerve tissue in the formalin solution for future immunofluroscent staining?

Badges
Science method

More Xue Deng's questions See All

Sample size calculation for multinomial logistic regression model?

May I know how to do Sample size calculation for multinomial logistic regression model?

10 November 2018 9,930 3 View

How to standardize coefficient in multinomial logistic regression model in SPSS?

(1). How to standardize coefficient in multinomial logistic regression model in SPSS? (2). After standardization of the coefficients in different multinomial logistic regression model, can we...

10 November 2018 7,796 0 View

How to quantify axonal loss without taking nerve biopsy but take nearby tissue in carpal tunnel syndrome?

I plan to take some tissues nearby while doing open release for carpal tunnel syndrome to quantify axonal loss of the injured median nerve. May I know any good laboratory techniques ? Thank you

01 February 2018 9,540 1 View

How to check multicollinearity of IVs in ordinal regression model using SPSS?

Dependent Variable (DV) is ordinal data. Independent variables (IVs) are continuous data. Using SPSS, Multicollinearity test can be checked in linear regression model. However, this is required...

11 December 2017 5,038 4 View

How to determine accuracy of predictors in ordinary regression model using SPSS ?

I have created an ordinary regression model with acceptable model-of-fitness, significant predictors, no sig. of test-of-paralelle line etc. Now I wish to determine accuracy of my predictors of...

10 November 2017 7,620 9 View

How to deal with irrational but statistically significant predictor in the model?

I have a dilemma when doing bootstrapping method to examine my model: I identified predictor A & B in my regression model as significant estimates. However, it can be rational only when (A...

10 November 2017 8,119 9 View

How is the performance of the ROC curve acceptable for publication?

I have sort out cut-off values of variables for differential diagnosis using an assessment tool. Although all the cut-off values are significant, however, the area under the curve (AUC) are from...

08 September 2017 9,690 2 View

Should I do PCR or Tissue assay in addition to IHC and ELISA for my research?

I wish to detect cytokine changes in the specific cortex in the rat brain. I am planning to apply IHC+ELISA for localization and quantitative analysis. Do I need to do PRC or Tissue assay to exam...

01 January 1970 9,958 2 View

How to perform multinomial scaling using SPSS?

Any guidelines for performing multinomial scaling using SPSS?A step-by-step guideline is preferred.

01 January 1970 8,199 0 View

With a poor performance in ROC curve of the model, does that mean the model is useless at all?

I created a binary model with results as follow: 1). Omnibus Test (Chi-sq.=31.705, p0.05) The model was statistically...

01 January 1970 4,040 5 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View