how long can i put my sciatic nerve tissue in the formalin solution for future immunofluroscent staining?
May I know how to do Sample size calculation for multinomial logistic regression model?
10 November 2018 9,690 3 View
(1). How to standardize coefficient in multinomial logistic regression model in SPSS? (2). After standardization of the coefficients in different multinomial logistic regression model, can we...
10 November 2018 7,648 0 View
I plan to take some tissues nearby while doing open release for carpal tunnel syndrome to quantify axonal loss of the injured median nerve. May I know any good laboratory techniques ? Thank you
01 February 2018 9,282 1 View
Dependent Variable (DV) is ordinal data. Independent variables (IVs) are continuous data. Using SPSS, Multicollinearity test can be checked in linear regression model. However, this is required...
11 December 2017 4,795 4 View
I have a dilemma when doing bootstrapping method to examine my model: I identified predictor A & B in my regression model as significant estimates. However, it can be rational only when (A...
10 November 2017 7,975 9 View
I have created an ordinary regression model with acceptable model-of-fitness, significant predictors, no sig. of test-of-paralelle line etc. Now I wish to determine accuracy of my predictors of...
10 November 2017 7,364 9 View
I have sort out cut-off values of variables for differential diagnosis using an assessment tool. Although all the cut-off values are significant, however, the area under the curve (AUC) are from...
08 September 2017 9,555 2 View
Any guidelines for performing multinomial scaling using SPSS?A step-by-step guideline is preferred.
01 January 1970 8,023 0 View
I created a binary model with results as follow: 1). Omnibus Test (Chi-sq.=31.705, p0.05) The model was statistically...
01 January 1970 3,875 5 View
I am going to test rat Hippocampus using ELISA. Before that, I wish to confirm if the tissue I am testing is hippocampus. So I am going to use H & E staining to exam the structure. May I know...
01 January 1970 5,467 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...
25 February 2021 6,635 5 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View