How long can I leave the ELISA plate (R&D DuoSet ELISA mouse IL-1beta) in capture antibody?

07 October 2022 1 5K Report

I am planning to coat the plate with capture antibody over the weekend at 4 degree. I am not sure if that will make a difference than incubating overnight at room temperature which is what the protocol suggests.

Has anyone extend the plate coating time using R&D DuoSet ELISA kit?

Malcolm Nobre

Hello Lulu Li

You may leave the ELISA plate in capture antibody for 24-48 hrs. at 4 degree C provided your coating protein is stable at 4-degree C. At the same time, you should bear in mind the risk of evaporation and contamination when the ELISA plate is kept for extended time point at 4 degree C, as these may affect the coating antigens and subsequently the end results.

Best.

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