Lutz protocol was adopted for BMDC (Balb/c BM) production,>95% CD11c positive, used for MLR. Could you share your experience?
GMCSF can be added for post-maturation efficiency of the cells, depending on what you want to achieve.
Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...
01 February 2021 6,756 4 View
Hi, everyone: I want to determine whether IL-2 and IL-15 have different effects on immunized CD8+ T cell effector function. In this study, I immunize WT mice with peptide on day 0 and 7, and...
02 December 2020 9,413 3 View
Respected researchers, I conducted a SEM including 5 variables (two independent variables , two mediators, and a dependent variables), the correlations between these variables range vary from...
01 December 2020 1,338 5 View
I have tried several different methods already, but each attempt ends with some error that basic troubleshooting can't surmount. I'm wondering if anyone has successfully converted Open Ephys data...
27 November 2020 1,279 2 View
I'm trying to test expression of a gene in sorted cells. I plan to extract RNA from sorted cells and to do RT-PCR (reverse transcription PCR). The problem is that from sorting, I can only collect...
21 November 2020 5,467 2 View
As the title has asked. For instence, using metal stick or long glass tube to get rid of the bubbles in columns.
05 November 2020 5,946 4 View
Hi, guys, Do you have problem using B cell negative selection kit form Meltinyi? Sometime the B cell is pure, CD19+>95%, but sometimes it lower than 90%.
19 October 2020 5,160 3 View
I want to do tissue subcellular fractionation of mouse liver. According to the method, the speed to separate ER is over 200,000g for 120 min (Song et al., 2006). However, liver exosome isolation...
12 October 2020 4,929 3 View
how to set up asphalt rheological model (CA and CAM) ? How to build EXCEL?
23 September 2020 7,692 2 View
Hi anyone experienced in studying antibody-antigen binding with biacore (SPR) know should 1:1 binding model be used in kinetics and affinity analysis? Or should we use 1:2(antibody:antigen)? Thanks!
26 August 2020 4,130 3 View
Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...
21 February 2021 5,724 4 View
I am doing a master in nursing but still does not have any idea about what topic I am going to choose. pls help
25 January 2021 1,097 12 View
Hello, I am an undergraduate student . Currently I'm working on "Hematological diagnosis using blood sample images " project . I am working on developing a deep learning model for this diagnosis...
05 July 2020 6,813 4 View
Hello everyone ! One of the most crucial concerns about food science is the expectation of development for GM foods,after this pandemic is over. Each opinion is respected and received. Your help...
29 May 2020 8,259 3 View
Biological techniques are methods or procedures that are used to study living things. They include experimental and computational methods, approaches, protocols and tools for biological research.
19 April 2020 4,247 26 View
Any synthetic analogue of Hemoglobin or any other Xenobiological agents can be cited as example. Please provide links and references.
20 November 2019 8,904 4 View
10 November 2019 2,970 4 View
Hey there, I'm using commercially available Drabkin's reagent to measure the hemoglobin content of blood photometrically. The reagent I bought contains sodium bicarbonate, potassium ferricyanide...
09 October 2019 1,497 10 View
I plan to conduct a study in which I will compare depression and quality of life among children with cancer to children with other chronic conditions (hematologic, kidney and respiratory chronic...
20 June 2019 2,916 6 View
05 June 2019 9,036 7 View