Alexa Wu

9 Questions 7 Answers 0 Followers

Questions related from Alexa Wu

What do you think about these human MSC I've cultured?

Hi! This is my first time to culture MSC(from MDS patients' BM), I notice there are three kinds of MSC: 1. stretched and big,irregular in shape,with sandy granule near nucleus, most frequently...

29 November 2015 1,491 7 View

How many ways there are to tell apart the cells of two origins co-cultured together?

Greetings! I have one kind of cell from two patients and I'd like to do a long-term(30 days) co-culture experiment with them. Then I'll have to seperate these two cell for the following...

02 October 2015 4,716 3 View

How long can BMDC last and should GMCSF be added after maturation?

Lutz protocol was adopted for BMDC (Balb/c BM) production,>95% CD11c positive, used for MLR. Could you share your experience?

18 December 2013 4,336 1 View

When should I add IL-2 for Th1 Th2 differentiation?

I'm currently working with MLR using murine cells (naive T co-cultured with BMDC). Should I add IL-2 along with other cytokines at the beginning, or add it later? Also, what concentration do you...

17 December 2013 5,158 1 View

Any recommendation for murine BM-DCs culture and culture protocol for mix lymphocyte reaction?

I'm currently using RPMI 1640 (plus 10% FBS, 55 μM 2-mercaptoethanol, 1mM sodium pyruvate,1mM HEPES, 1mM NEAA,1% antibiotic-antimyetic ) for BM-DCs and MLR. Any suggestions to improve this...

12 October 2013 6,328 2 View

Can someone advise on massive cell apoptosis observed after radiation?

Murine BM-DCs ( induced from C57 BM cells with GM-CSF and IL-4 for 7days plus LPS on day 7), 25Gy was applied but massive cell apoptosis was observed (Annexin V+ 7AAD+)afterwards (same case with...

12 October 2013 6,814 4 View

Is it right to make a mix of all media supplements before adding to the basic culture media?

I'm using 10%FBS RPMI 1640 with 2-Mercaptoethanol, Sodium Pyruvate,HEPES, Non-Essential Amino Acids and Antibiotic-Antimycotic, can I make a mix of all these media supplements prior usage and...

07 October 2013 2,659 3 View

Is IL-2 needed in Th1 and Th2 induction?

Typically IL-12 plus anti-IL-4 are needed in Th1 induction from naive T cells and IL-4 plus anti-IFN-gamma for Th2, but what about IL-2? Is it also necessary for T cell differentiation?

07 October 2013 6,688 5 View

Is cell morphology a reliable proof in deciding whether BM-DC is mature or not?

BM cells was harvested from B6, cultured in RPMI 1640 or DEME with 20ng/ml GM-CSF and 20ng/ml IL-4 for 6 days (seeding density was 1x10^6), 1μg/ml LPS was added on day 7, however, adherent cells...

07 October 2013 9,836 8 View