Data suggests that unprocessed tissue specimens should not be stored at temperatures of −20°C for greater than one month. However, please note that such storage for even shorter periods is probably not advisable.
Animal tissues can be stored at \(-20^\circ\)C for up to 3 months with minimal degradation. For longer storage, up to 6 months, some degradation may occur, and beyond that, \(-80^\circ\)C or lower is recommended to preserve tissue integrity.