How is the inter- and Intra-assay coefficients of variability (%CV) calculated in the microplate reader (ELISA)?

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Why there are no peaks at downfield NMR of Cobalt and Nickel complexes?

I synthesized some paramagnetic cobalt and nickel complexes (expected to be Co(II) and Ni(II)) using a bidentate ligand - N of pyridine ring (aromatic) and O of P=O (aliphatic), and I obtained...

03 July 2024 3,410 4 View

Why does the static pressure at the outlet become a very negative value when the outlet flow boundary conditions are used?

The simulation is for turbulent and incompressible flow and the inlet velocity condition is selected for the inlet

12 June 2024 3,568 2 View

What is the best method to enhance the surface hardness of cutting tools made from SPK steel?

SPK tool steels are used for cutting wires made from low carbon steels. We are searching for a cheap and effective method for increasing the cutting characteristics of the cutters.

14 May 2024 4,948 0 View

Hello, does anyone know of any calcite marine organisms that have a growth rate of about 1 micrometer per day?

I am looking for examples of organisms where calcite growth rate occurs at a rate of approximately 1 micrometer per day, or any paper.

16 April 2024 9,752 0 View

What methods and indicators are the most important methods in investigating the neotectonics of a watershed that can be used as a global standard?

14 February 2024 1,669 3 View

What form of silica and particle size are suitable for fostering the growth of Diatom microalgae?

What form of silica and particle size are suitable for fostering the growth of Diatom microalgae? Can nano-silica be employed in the context of promoting Diatom growth?

19 December 2023 964 3 View

FRICTION BUFFER-STOPS (friction jaws)?

Hi I want to modeling FEM of friction stopper. Can anyone tell me about the materials of friction shoes? I know that their made from a bronze and phosphorous alloy. I need more information....

11 December 2023 10,023 2 View

Degree in which amorphous halo appeared in XRD patterns?

I just performed a XRD analysis on a metallic glass particle and got an amorphous halo around 2(theta)=44 degree. How can I justify why the peak appears around 44 degrees?

20 November 2023 5,205 1 View

Regarding sex determinant in birds by Real time PCR. How can do it?

12 May 2023 5,899 3 View

How to code if condition in LINGO?

hi...sorry i have one problem to write if condition in LINGO, can anyone help me to code this simple if condition?

07 May 2023 2,337 8 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View

Why Orca calculations take too loong?

Hello! I am trying to calculate the sumatriptan molecule, but it has been counting for five days and it does not stop. It seems to me that it is too long and I am doing something wrong. Please...

25 July 2024 3,364 5 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View