How is the inter- and Intra-assay coefficients of variability (%CV) calculated in the microplate reader (ELISA)?

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What reference do you recommend about doublet lattice method (DLM) coupling with finite element method (FEM) ?

I want to write a code related to the flutter of aeroelastic wing under unsteady load using doublet lattice method (DLM). I want a good reference with numerical examples or a book which learn me...

25 January 2021 7,488 3 View

Is it possible to culture several cell lines in the same plate/dish/flask with complete cell separation?

I'm trying to screen the effect of a toxic agent. I need to see its effect on a few different cell lines with different degrees of resistance to this compound. I would like to possibly culture...

31 December 2020 5,740 3 View

Difference between electro-optical switches and modulators?

Hi Suppose an electro-optical structure that by applying an external voltage, the output light can be in the ON or OFF states. The structure has one input port and one output port. Now, Is this...

10 December 2020 8,276 7 View

Article about translation?

Hello I need 2 articles in translation field and for last 5 years, just for getting the methodology of them and write the way of collecting data and the way of their analysis , for our...

09 December 2020 541 5 View

Is there any munimum sample/group size in ANOVA?

Hi , We are investigation whether Syaesthesia score across has a significant difference across the four groups av individuals (male/female respective high/low in opennennss to experience...

20 October 2020 2,623 3 View

Not computed observation wells in MT3D?

Hello, I created MODFLOW and MT3D models in GMS for a real plain. (Quantitative and qualitative model) In quality mode (MT3D), some of the observation wells have been computed to be zero! While...

13 July 2020 5,053 4 View

Hi everyone, where can I find an updated dataset of hotel reviews?

I'm looking for a dataset comprises of reviews, ratings, reviewer nationality, name and place of the hotel, and so on.

08 July 2020 2,680 4 View

What references do you recommend for Machine Learning and its applications in Mechanical Engineering?

I want to study Machine learning and its applications in structural mechanics, Can anyone introduce a straight forward reference in simple method?

14 June 2020 9,517 4 View

Over the past 6 months, the number of patients with Covid -19 in China has not increased , but the number of cases in other countries to rise, Why?

Over the past 6 months, the number of patients with Covid -19 in China has not increased , but the number of patients in other countries to rise, . What do you think about this? What is the reason?

04 June 2020 5,755 84 View

How can the quantum resonance degradation process be used indefinitely to disinfect the environment and anti-pandemic COVID-19 ?

In the name of God I would like to thank the doctors, nurses, medical staff and those who are with the people during these difficult days. Anti-Pandemic COVID-19 : How can the quantum resonance...

26 May 2020 8,370 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View