How to deal with negative values of autoradiography scans when plotting a calibration plot?

I am quantifying spots on an autoradiography scan, so subtracting the background pixels to get the intensity values but when nothing binds, it's the same as the background and since that's an...

18 August 2020 3,265 4 View

How to find/predict transcription factors for a putative promoter of the gene of my interest?

Dear All, Based on ORCA conserved regions, CpG islands, and histone methylation, I predict a putative promotor for my gene of interest with one of my colleague's help. I need to find/predict...

21 February 2020 3,035 3 View

Can a 150KDa antibody pass through 100KDa filter at 15,000g for 1 hour?

Can a 150KDa antibody pass through 100KDa filter at 15,000g for 1 hour via centrifugation?

30 September 2019 9,592 3 View

Can you fill a shell volume with SPH in LS DYNA?

Hello All! I'm finding it hard to fill the attached keyword shell volume with SPH elements. Can anybody tell me why? Is it because of the complex geometry? Thanks in advance

25 September 2019 6,886 1 View

Strain Rate in LS DYNA user defined materials?

Hello All! Anybody knows a better way than eps(i) / dt1 to calculate strain rates in a UMAT? I'm trying with eps / dt1 but apparently it is not giving me a proper rate-dependent result. Thanks..

16 July 2019 3,700 7 View

Software other than ANSYS and MCalibration to get Mooney Rivlin Constants?

Hello All! I am using both ANSYS and MCalibration to get model constants for a 9 parameter Mooney Rivlin model using my uni-axial tension test data. These two software give me extremely different...

14 May 2019 352 6 View

Good protocol for Mouse ESC electroporation?

Dear all, Would you kindly share with me a good protocol for mouse embryonic stem cells electroporation using Neon Transfection System? What parameters and plasmid concentrations that I should...

20 January 2019 9,045 9 View

I need a clarification regarding reverse coding of negatively-keyed items in a likert scale?

If a questionnaire includes positively and negatively-keyed items, 1)should all the negatively keyed items be reverse coded? for example, if there are two items, 1. I like the game 2. The game is...

13 September 2017 4,955 10 View

How to analyze MCQs and open-ended questions using SPSS?

30 July 2016 6,920 9 View

Can anyone help with counting cells with fluorescence?

Hi! I have a question about how to determine cells with fluorescence in a given image (transfection efficiency calculation ). For example under a given set of conditions (expose time) cells in an...

27 July 2014 707 5 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View