We are looking into how we can characterize focal adhesion that may indicate migration--either enhancement or impairment. A paper shows Gaussian correlation. I am not sure how established these observations are. We are aiming for characters that we can measure through fixed-cell imaging since live-cell is often harder to deal with.
Thanks for the help.
Hi,
If you are using a confocal microscopy, you are able to get images through the sample; so, the migration is very clear inside the tissue. Blocking the samples in paraffin and then cross-sections also can show the cells migration with histochemical stainings.
Yes they are guassian. That is too little or too much adhesion affects motility negatively. You want transient attachment and extension of protrusions. Establishment of dense stress fibers usually leads to impaired cell migration. You can check some of the papers published by Anna Huttenlocher.
I also had a paper few years back that touched on this a bit.
http://jcb.rupress.org/content/199/6/951.long
Hi Jonard, focal adhesions are a complex mix of intracellular proteins that transfer integrin binding to the internal cytoskeleton.
Many integrins bind to an RGD sequence which means that this binding is weak. Integrins have to have intracellular support to function in adhesion and migration. My advice is to colocalize integrins with focal adhesion proteins. (paper included)
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