I bought a non lysine-fixable FITC-Dextran70KDa and wanting to trace blood vessel formation in vitro. I am wondering if I can do microfluidic perfusion after I fix organoids and organs (say, lungs). I wanna test some fixed whole organoids and organs that we already have in the lab. Thanks in advance for answers!
Hi Jonard! Did I read it correctly, you want to use non-lysine-fixable dextran on already fixed organoids?
In our case, we use FITC-dextran to view endosomes within living cells. Whenever we have dextran uptake experiments that require fixing cells, we have to use lysine-fixable dextran (https://www.thermofisher.com/order/catalog/product/D1845 ). Non-lysine-fixable dextran tends to "leak out" of cells even when using fixatives (i.e., paraformaldehyde PFA). Lysine-fixable dextrans can be crosslinked with the PFA and can remain on its location.
In your case (if Iu understood it correctly), you are using pre-fixed samples. If you have no plans to fix these once more, then non-lysine-fixable dextran is enough. But if you want to treat these samples with dextran and want to keep the signals on its place, I would recommend using lysine-fixable dextrans.
I hope this helps! Good luck! :)
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