In my study of biomass pre-treatment, I have carried out acid hydrolysis using Sulfuric acid. After separating the hydrolysate, I am checking the amount of fermentable sugar released. As from the literature, acid pre-treatment works on only the hemicellulose fraction. But can there be glucose monomer too ??
To have clear account of the amount of sugar released.
1. Orcinol method for only pentose sugar released (as suggested to other Research gate followers)
2. DNS method for Total reducing sugar. (absorbance at 540 nm against standard Glucose)
How can I have an idea of the efficiency of the treatment, in terms of pentose and hexose (if any ??) sugar yield.
Please suggest
Hi Pranab
for sugar analysis you can use Dubois 1956 a colorimetric method, also named phenol/sulphuric method. See the original paper.
Hi,
Parnab
You should analyze the sugars content of water soluble and water insoluble portions. You can use either calometric methods or HPLC based analysis.
two type of acid hydrolysis
1. dilute acid hydrolysis for hemicellulose converted into xylose (pentose). find attachment for estimation of xylose by the p-bromoaniline method.
2. concentrate acid hydrolysis for cellulose converted into glucose ((hexsoe). glucose estimation by a Kit accurex.
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