Pranab Kumar Nath

6 Questions 12 Answers 0 Followers

Questions related from Pranab Kumar Nath

Is it possible to prepare plant biomass sample for liquid state NMR?

I have access to NMR instrument which is used only to assay samples in the liquid state (dissolved form). Kindly suggest any protocol. I have access to a few literatures which says a mix of ionic...

19 July 2017 2,787 3 View

Should I consider the volume of DNS during FPase activity calculation?

Hi everyone, For my cellulase activity calculation, I have added 1 ml crude enzyme + 1 ml Buffer with filter paper. After incubation, added 3 ml DNS, heated the tubes and took reading. What will...

27 February 2017 3,436 2 View

How do I filter alkali hydrolysate from pellet ?

Dear All, I have been working on chemical pretreatment using acid followed by alkali. I am having difficulty filtering the alkali hydrolysate (which is very much pulpy in nature) by vacuum filter...

25 May 2015 2,450 7 View

How is the efficiency of acid treatment for pentose and Hexose yield assayed?

In my study of biomass pre-treatment, I have carried out acid hydrolysis using Sulfuric acid. After separating the hydrolysate, I am checking the amount of fermentable sugar released. As from the...

25 April 2015 4,508 4 View

How is alkaline peroxide treatment carried out?

Is it the application of H2O2 in the presence of alkali? What are the parameters that can be checked in Alkaline peroxide pretreatment of biomass?

17 December 2014 6,058 2 View

Does acid treatment of biomass signify pretreatment or saccharification/hydrolysis to sugar monomer?

In most research papers, the ground biomass is first treated with acid, then with alkaii and subsequently saccharification by fungus or commercial enzymes. How can we categorize the treatments...

15 November 2014 414 8 View