How is lysis buffer for DNA extraction made?

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How to monitor surfaces current on Waveguide Problem with CST Software?

Hello, everyone! I've placed an FSS (dielectric and FSS) inside a waveguide, but I'm unable to detect surface currents on the probe inside (the distribution, as monitored by CST, is confined...

27 April 2024 8,651 0 View

Why is my AKTA Pure showing protein concentrations lower than expected?

I've recently purified TEV with a his-tag on a Ni-NTA column using our AKTA Pure FPLC. It usually works great. This time I had two peaks of lower absorbance over many fractions in the elution...

27 August 2023 7,415 0 View

How to monitor RCS on CST?

Hello everybody, I would like to know how to monitor RCS on CST. Do someone have any tutorial about this kind of topic?

13 August 2022 4,780 4 View

Why is my Bradford Assay absorbing at 660 nm?

I have been modifying a protein through a radical reaction, which conjugates to tyrosine. As such, measuring protein concentration by absorbance at 280 nm is not working anymore. So I was told,...

14 June 2022 5,090 4 View

How can I Set TE/TM mode in CST ?

Hello everybody, I try to be clear for what I am looking for. I would like to set TE and TM mode varying the angle of incidence. I know that varying teta I could modify incidence angle, and it is...

31 May 2022 9,959 1 View

How can i modify the images in my manuscript?

Ricci V, Ricci C, Gervasoni F, Andreoli A, Özcakar L. From histo-anatomy to sonography in lymphedema: EURO-MUSCULUS/USPRM approach. Eur J Phys Rehabil Med. 2021 Apr 16. doi:...

10 January 2022 5,186 0 View

How is it possible to choose k in KNN algorithm in a unsupervised dataset?

Hello to everyone. I am trying to implement KNN analysis to fix minPts in the DBSCAN clustering algorithm. My dataset is composed only of 4 variables and 935 observations. I have found that if k =...

09 January 2022 5,450 4 View

How can I remove DNA from lysate?

I am working with a protocol that requires cell pellet resuspension in 6 M guanidinium. I did not make this protocol, so I don't know how they came to this optimization. I keep getting very...

08 December 2021 6,867 2 View

Why the buckling analysis with Ansys beam188 element and Kop3=2 does not work?

I try to do buckling analysis with Ansys using beam188 element; if I set the K option 3 = 0, it works, but when I set Kop3=2, it fails to get the eigenvalues. A message informs that file of...

12 October 2021 5,970 2 View

How do I get my protein back after a leak in dialysis?

I'm training an undergrad and they didn't clamp the dialysis bag correctly. All the protein leaked out in the dialysis buffer. I now have my 15 mL sample floating around in 2 L of buffer. The...

14 April 2021 9,649 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View