Hello to fellow researchers,
I would like to view DNA using AFM. What I want to look at more specifically is 300nm long double stranded DNA.
In AFM, samples can be visuallised either in solution or in air. I want to know sample preparation for DNA in each case.
Since I have never done AFM or just known that it is easier to prepare samples than TEM, which is the technique I performed before, if you could elaborate extensively, that would be great.
I also have the doubt whether 2nM thick double-stranded DNA can be 'effectively' or 'clearly' visuallised in AFM (maybe, old AFM does not offer good resolution)??
Thank you~~~
OK, I won't be able to give you a final answer, but here are some thoughts:
-you should elaborate on the questions you want to answer with your experiment. If you just want to see that DNA is there, you can easily coat a wafer out of your solution, evaporate the solvent and you should see the strand.
-on the other hand, if you want to resolve the internal structure of the strand, that won't be so easy because you'll need close-to-atomic reolutions which will probably require a UHV-AFM (cryosetup might be helpful) plus a functionalized tip.
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