How is chemical quantification of graphene done?

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How to calculate the charge densities of valence band maximum (VBM) and conduction band minimum (CBM) of a system using Quantum ESPRESSO software?

Hello everyone, I am using Quantum ESPRESSO software to calculate the total charge density of the compounds. However, I am unable to calculate the charge densities of valence band maximum and...

06 June 2024 4,498 4 View

How is object segmentation and object detection are different?

How YOLOv8 is used to obtain the contours/object and calculate individual area

02 May 2024 5,396 3 View

How to get rid of "error termination in SCF gradient" in ORCA 5.0?

I am optimizing a small organic molecule in a triplet second excited state using TD-DFT in ORCA 5.0. It is giving error termination in SCF gradient. How to get rid of it? Check the error below....

30 April 2024 6,806 6 View

How can I get my desired DNA copies?

Hi all, I want to have 301 copies/ul for my downstream experiments. The sample I am using is a purified plasmid, of which I know the concentration (ng/ul), volume (ul), and molarity(g/mol). How...

20 April 2024 2,468 4 View

Is it good to have antibiotic resistance property for probiotic?

To prevent intestinal disorders, probiotics can be rendered multidrug-resistant to survive in the presence of co-administered antibiotics, and they may also transmit the antibiotic-resistance gene...

01 April 2024 8,043 2 View

After formulating your hypothesis based on a problem statement , what are the important steps constructed within DS project life cycle ?

Please explain the importance of : 1. DOE - Design Of Experiment 2. Statistical Testing Process Document

12 March 2024 1,556 1 View

Can anyone suggest me how to ensure proper revival for the strain B. braunii UTEX 572?

I have bought strain B. braunii UTEX572 for my work, however, I do not see any positive growth after reviving it in both Bold 3N liquid and agar media supplemented with 10g/L glucose. Is there any...

10 March 2024 4,196 0 View

Could you tell me the cause of this problem?

A person has very little thirst, dry mouth. If he drinks water, the water is coming out through the urine, but his body lacks water. Why does this happen and what is the remedy?

16 February 2024 1,255 3 View

How to make agar plates using the drug 5-fluoroindole as it is light sensitive and sparingly soluble in water?

I need some suggestions on working with the drug 5-fluoroindole. I am working on green microalgae and trying to make my strain tryptophan auxotroph. However, I am unable to make proper drug plates...

14 February 2024 1,013 1 View

How to remove antibiotic markers from the microalgal genome post CRISPR-RNP based knock-in?

When CRISPR-Cas9 RNP-based gene knock-in is performed in green microalgae, the donor cassette will contain the GOI and some selection marker genes. Post the gene integration how do I remove the...

04 February 2024 6,667 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View