Hi to all I immobilized a peroxidase on a new synthesized MOF. The reviewer of a journal paper asked me to consider the inhibitory activity and specific affinity of my immobilized enzyme in the introduction. How I can answer this comment of the reviewer for a journal paper revision? What does it mean? In my opinion, these items are related to biosensors. I reported the influence of immobilization on km in my article but I have no idea about the inhibitory activity.
i think that the reviewer is meaning the comparison between the free and immobilized enzyme in term of Km , because it define the affinity of the enzyme , also the comparison of Vmax
Nassima Tandjaoui
Thanks for your response but I report these items...
The specific activity of an enzyme preparation is the turnover-number (molecules of substrate that an enzyme molecule handles per second) multiplied with the enzyme concentration.
What your reviewer is in effect asking is
- whether the enzyme might have been partially damaged during immobilisation
- whether there is any steric hindrance to substrate binding/product release due to the immobilisation.
Any such effect could invalidate your observed Km. However, I am not sure that this belongs into the introduction. Evidence of full activity should be presented under results, and its significance in the context of the paper discussed in the discussion section. An exception would be IMHO if the immobilisation procedure is well established and proven to result in fully active enzyme, then this would be mentioned in the background part of the introduction, with appropriate references. Of course, you'd still have to verify that your preparation is fully active, and this should be mentioned under results.
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