Soudabeh Farhadi

7 Questions 4 Answers 0 Followers

Questions related from Soudabeh Farhadi

How I can isolate after centrifuging?

Hello everyone I Load apolipoprotein on extracellular vesicles (EVs). After centrifuging, I have EVs, apolipoprotein, and also loaded apolipoprotein. How I can separate them? And also confirm...

25 October 2022 5,005 1 View

How sulfide concentration is measured by standard spectrophotometric method?

Hi dear researchers In a nature report "Amperometric inhibitive biosensor based on horseradish peroxidasenanoporous gold for sulfide determination" measuring sulfide concentration in a wastewater...

19 November 2017 8,451 3 View

How inhibitory activity and specific affinity related to immobilized enzyme?

Hi to all I immobilized a peroxidase on a new synthesized MOF. The reviewer of a journal paper asked me to consider the inhibitory activity and specific affinity of my immobilized enzyme in the...

01 January 1970 3,420 3 View

Why when I use commercial HRP and TMB as substrate, not giving expected blue color?

Hi dear researchers We used HRP (Biobasic) in phosphate buffer (pH: 6.5) and after adding TMB, the reaction turning to light blue without adding H2O2. After adding H2O2 to reaction media, the...

01 January 1970 850 3 View

How can I determine pH stability of my immobilized enzyme?

Hi researchers I determine optimal pH and temperature of immobilized enzyme activity. I want to know how pre-incubated it at different pH for pH stability factor.

01 January 1970 6,581 4 View

What is the difference between enzyme immobilized activity yield and recovery?

I am studying enzyme immobilized nanoparticles for catalytic applications. Research are using different names like activity yield or activity recovery. What is the difference between enzyme...

01 January 1970 2,849 12 View

What is your suggestion about the best protocol for differentiating rat adipose-drived stem cells into cardiomyocytes by azacytidine?

Dear researchers, I hope you and everyone in your circle are doing well and healthy. I’m going to work on some factors that affect on differentiation of cardiac progenitor cells to...

01 January 1970 6,022 4 View