You can investigate the physiological and developmental responses of Brachypodium (eg, soluble protein, amino acids and chlorophyll leaf concentrations - and even leaf growth) at different levels of N, thinking of the plant fitness improvement. See Bradford (1976), Lichtenthaler (1987) and Puiatti & Sodek (1999).
I have germinated Brachypodium in petri dish for more 48 hours after I soaked it for 20 hours, but I have not got on germination in my seeds. How I can to got on a good procedure to that?