How I can slice rat`s cerebellum in a good way?

25 October 2021 1 10K Report

I´m using Leica VT1000 vibratome for slicing rat´s cerebellum for patch clamp experiments. Which parameters are better to use?

Thanks

Matthew Xu-Friedman

Back when I worked on cerebellum, we used the maximum amplitude, and a slow advancement speed (0.05 mm/s). The thickness depends on what you can handle (e.g. 200 or 300 um).

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