How I can separate out total IgG, IgM and IgA from a plasma/serum sample so that each fraction can be used for an assay?

More Rommel Iheozor-Ejiofor's questions See All

N-terminus cleavage site prediction?

I am trying to predict if a particular protein can cleave a receptor at the N-terminus using in silico techniques. Is this possible?

30 April 2023 5,904 0 View

Could you please help me respond to my dissertation research questionnaire?

Hi everyone. My name is Judith Ejiofor. I am a Master's students at De Montfort University, Leicester. I am doing a research on "The Moderation Role of Percieved Risk: The Relationship between...

19 April 2023 7,878 2 View

Why MaxQuant doesn´t show processes in the "Performance" window after "Start"?

I'm analyzing 42 .mzXML files (each less than 200 MB of size) in MaxQuant 2.0.3.0 in a good computer (32 GB RAM, Intel® Xeon® Processor E5-2687W v2, 3.4 GHz 8 cores 16 thread CPU [2 processors]),...

17 November 2021 5,494 3 View

Why MetaboAnalyst 5.0 doesn't detect my raw data as Centroided?

I'm currently trying to analyze .mzXML MS-data in MetaboAnalyst 5.0 Raw Data Processing pipeline, but some files aren't detected as "centroided", even though I converted all my files from...

21 June 2021 3,315 1 View

How could someone measure or estimate the amount of CO2 absorbed by vegetation, land and water bodies in a country?

most often we measure the dynamics of co2 emission without recourse to the amount absorbed in the system. so, i am working on how to lessen the burden of those absorbed by land, sea, vegetation...

27 October 2018 9,798 3 View

What are the goals of doing a comparative study of different shortest path algorithms?

For our research topic, we plan on Comparing shortest path algorithms use in route planning by stressing them under different scenarios. Our goal is to contrast the algorithms and see which is the...

06 February 2018 8,340 0 View

Aside from djikstra's shortest path algo, what are other effective algo used in route planning? And why?

Are there any algorithm besides Djiktra's algorithm used in route planning which factors not only the shortest distance, but also traffic and such?

21 January 2018 3,699 5 View

What effect does vitamin C have on artemisinin when co-administered together in malaria infection?

Please, can anyone assist me the answer? thanks

15 October 2017 7,246 5 View

How does Haem activate the peroxidase bond in artemisinin in malaria infection?

Please, I want in vitro experts, Molecular Biologist, and parasitologist to assist me the the answer. Thank you

15 October 2017 4,764 1 View

Please give me a list of commerical laboratories with HPLC System, where i can identify phenolic compounds in my plant extract

I need laboratories when i can determine the phenolic compounds in plant extract using HPLC.

17 May 2017 2,387 7 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Help on understanding the implementation of Mori Tanaka method on MATLAB?

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30 July 2024 969 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

ELISA sandwich with two monoclonal antibodies ?

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16 July 2024 9,044 10 View

Not able to give GAFF forcefield to my ligand?

I have been able to assign the CHARMM forcefield to my ligand i.e. acetyl coenzyme A but not the amber forcefield. I want to run amber forcefield and the parameterization of ligand is failing. I...

15 July 2024 7,323 1 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

The HCP removal rate is lower than usual in concentration step, why?

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Shen-An Hwang

There are commerical columns/products for antibody purification. Below is just one example.

https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/antibody-purification-column-formats.html

Maurice Ekpenyong

Please follow this link;Article A One Step Separation of Immunoglobulin G from Bovine Serum ...

and https://core.ac.uk/download/pdf/82378354.pdf

Most importantly, read the attached article. It's been very helpful.

Baydaa A. Hassan

can be used ELIZA Technique

Rommel Iheozor-Ejiofor

I forgot to mention that I have an average of 50 microliters of serum. The samples are precious.

I still think your best bet is still the commerical spin columns...

Or if you can give more details on what assay you want to do.. maybe someone can come up with a way where you don't need to separtae the antibodies.