I have a serum sample and plan to do some assay using IgG, IgM and IgA fraction of the serum. I know I can precipitate IgG out using Gullsorb but the precipitated IgG will be useless to me afterwards. Any ideas would be highly appreciated
There are commerical columns/products for antibody purification. Below is just one example.
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/antibody-purification-column-formats.html
Please follow this link;Article A One Step Separation of Immunoglobulin G from Bovine Serum ...
Most importantly, read the attached article. It's been very helpful.
can be used ELIZA Technique
I forgot to mention that I have an average of 50 microliters of serum. The samples are precious.
I still think your best bet is still the commerical spin columns...
Or if you can give more details on what assay you want to do.. maybe someone can come up with a way where you don't need to separtae the antibodies.
Excerpt from https://en.m.wikipedia.org/wiki/Mach%27s_principle :
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