The best type of bioassays choice and non choice for arthropods for indicate preference for plant varieties of same species. Four varieties and a phytophagous.
Dear you can rear the insect which you want to use or check the host preference, and now grow new seedling for this experiment under laboratory condition and then apply the insect and evaluate the host preference.
For this purpose you must rear the insect up to five generations and then you able to evaluate.