An assay is an analytical bit by bit laboratory procedure for qualitative or quantitative evaluation of the presence, amount, or functional activity of an analyte. A protocol is a set of rules for a situation, whereas a method is a rigorous set of rules more rigorously defined than a protocol. However, in practice the words are used interchangeably as said by Paul.
An analytical method is a collection of set points required to run a single sample
An assay is an analytic procedure followed in laboratory for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity
A lab protocol, also known as a standard operating procedure, is a list of instructions to perform an experiment.
An assay is an analytical bit by bit laboratory procedure for qualitative or quantitative evaluation of the presence, amount, or functional activity of an analyte. A protocol is a set of rules for a situation, whereas a method is a rigorous set of rules more rigorously defined than a protocol. However, in practice the words are used interchangeably as said by Paul.
Assay is quantitative determination of content of analyte. Assay of analyte can be determined using chemical technique like potentiometric titration or using HPLC or GC chromatography etc.
Method is combinations of different methods which used to determine the various parameters of analyte.
Protocol is documents which designed what studies need to perform for particular test.