What will happen if someone to be in darkness for 10 years?

Physiological changes

08 September 2014 629 1 View

How to clone and express the desired gene in plants using Agrobacterium rhizogenesis?

I am planning to express and produce the desired protein in hairy root culture. Can anyone help me in the selection of vector and other requirements?

01 February 2014 6,408 3 View

Why was the enzyme having higher activity after adding inhibitor?

I have tried plate inhibitory assay for the identification of inhibitors. After adding one particular compound, the zone size was increasing three fold than control. What is the mechanism behind that?

01 February 2014 8,755 3 View

How does the secondary metabolic status change in protoplast fusion plants?

I am curious to know about the secondary metabolism of protoplast fusion plants (Different species Ex. One plant from Fabaceae and another one from Asteraceae). Is it possible to do this and how...

01 February 2014 4,475 9 View

What is the best way to approach PDF in Europe?

Post Doctoral Fellowship

31 December 2013 6,659 4 View

What are the elicitors used in the enhancement of flavonoid in callus culture?

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11 December 2013 1,547 3 View

How to prepare preparative TLC plates?

I have prepared TLC plates using Silica gel G (Merk). I could not even get the surface. Can anyone help me?

10 November 2013 9,622 10 View

Can anyone tell me about the recent development in chromatography techniques?

i.e. Isolation of metabolites from natural sources.

10 November 2013 6,969 0 View

How does one prepare the crystals from plant extracts?

Can anyone tell me about the crystal growth procedure from plant extracts?

09 October 2013 4,557 10 View

Can anyone tell me about RNAi in plants?

I am planning to do RNAi in plants. What are the criteria I should consider before starting my work?

09 October 2013 4,326 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View