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Questions related from Subramaniyam Ravichandran
Hello all, I have been running sequencing gels for quite some time now and I find that the bands start broadening as the dye reaches the halfway mark (pic attached). I followed the publications...
11 November 2018 9,267 9 View
Hi all, I purchased the SH-SY5Y cell line from ATCC (CRL2266). When I try to grow them in DMEM:F12 (1:1) media, the cells grow well for 3 days and then completely detach. I have also observed the...
05 May 2018 7,309 10 View
Hi all, I am performing a Co-IP experiment where would like to check the binding of my protein to the DNA of interest. I kept a control where I incubated the DNA and antibody only with the...
05 May 2018 862 4 View
I would like to calculate the concentration of T7 polymerase in the enzyme from NEB. Can anyone tell me how I can do this? Thanks in advance.
04 April 2018 6,851 4 View
Does TON in catalysis have any units? I have seen overlap of TOF and TON (TOF=TON/t), but papers seem to be using s-1 for both. I will be grateful if anybody can shed light on a proper...
10 October 2016 1,918 5 View
I am conducting 1H NMR experiments on a 700 MHz machine to analyze DNA-chemical binding. The chemical I am using is an intense blue color and I have used a sub-saturation stoichiometry for the...
03 March 2016 2,306 6 View
I have a fwd primer with 37% GC content and the rev primer with 61% GC content. The Tm of the fwd primer is 55.6 C and that of the rev is 58.2 C. Upon performing gradient PCR, I saw bands at 50C...
12 December 2014 4,523 9 View
Since both N and C terminals are needed for dimerization they cannot be tagged, I presume. My protein forms N-C dimers that are required for its activity. Any truncation or modification could lead...
10 October 2014 3,885 4 View
