How has otherness added to the monstrosity of the creature in Frankenstein and Heathcliff in Wuthering Heights?

02 January 2024 0 2K Report

What approaches did Shelley and Bronte use in developing their characterizations of the two given the historical events of the time periods and the limits they faced as women?

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Experts on orchid viruses for an article for home gardeners?

I am writing an article for my blog regarding orchid viruses aimed at home gardeners. It will include details of home testing kits their use and accuracy. I also want to include details about...

26 June 2024 7,422 1 View

Lentiviral Transduction Issues?

Hi there, I am attempting to transduce cells with the TLCV2 plasmid containing our sgRNA of interest. My transfection of the HEK 293T cells seems to yield positive results as the EGFP is clearly...

06 June 2024 2,131 1 View

Job Evaluation - what is the key advantage / key disadvantage of point-factor rating?

I am look at Job evaluation and it feels that the advantages of point-factor rating seems to also be the disadvantages - for example it is objective, but also subjective

14 May 2024 4,747 0 View

Which questionnaire can be used to investigate which leadership style an employee prefers?

For my thesis, im doing research in finding the leadership style that is the best match with organization X to maintain work engagement. For this research I'm going to interview 10 employees and...

19 March 2024 539 0 View

Who is researching trauma right now, specifically childhood trauma?

Hi! I am graduating with my masters in Marriage & Family therapy the end of February & want to continue my education with a PhD. I applied to Florida State but that did not work out. What...

07 February 2024 3,341 2 View

Which LAMP kit works better: NEB or ThermoFisher?

Hello, I'm currently in need of performing Loop-Mediated Isothermal Amplification (LAMP). There are modifications in both the Bst DNA polymerases from NEB and ThermoFisher. Has anyone had...

31 January 2024 2,534 1 View

What is recent development/algorithms in elliptic curve cryptography for image encryption?

Image and text encryption

26 January 2024 1,223 4 View

Is anyone aware of a set of accessible anonymised free text clinical records related to primary care?

I am looking for access to some publicly (or cheaply) available free-text clinical records, to help with the development of an AI tool. I am aware of CPRD (too expensive, not free text) and...

15 January 2024 7,535 1 View

Prostate Cancer Cell Lines from Black / African origin patients?

Hello all. We are trying to get access to prostate cancer cell lines that were derived from patients who were Black or of African descent. There are numerous relevant cell lines reported in the...

07 December 2023 4,969 3 View

What type of sample prep is needed for SDS-PAGE electrophoresis of plasma?

I mixed EDTA mouse plasma with standard gel sample buffer and got very smeary image on Western with no distinct bands. Is there something in the plasma that needs to be removed before I run gels?

06 December 2023 8,966 1 View

What condition of Kirat Rai's participation in politics before and now in Nepal?

The political participation of the Kirat Rai community in Nepal has evolved significantly over time: Historical Context Historically, the Kirat Rai people, like many indigenous groups in Nepal,...

05 August 2024 5,950 1 View

Challenges Faced by Freshers in IT Sector Recruitment?

i need to know what the key factors that the freshers are facing to get into the recruitment process, explain me clearly

31 July 2024 6,872 2 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

What are the most effective strategies for integrating sustainability in your expertise?

What challenges do you think businesses or entities face in implementing these strategies? Thanks

28 July 2024 2,125 14 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

I have two problems: 1) the enzyme is not immobilizing efficiently into the MOF material.. 2) the MOF itself has peak on 400nm by using p-NPA test.?

I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...

20 July 2024 1,440 3 View

What is the best method to conserve the medicinal plant facing rapid decline in population ?

I have identified specific medicinal plant facing rapid decline in population due to population pressure, overutilization, unsustainable harvesting and limited distribution. What can be the best...

15 July 2024 6,599 2 View

Psychological Theories on the problems faced by people who are 'Bicultural'?

Theoretical framework

15 July 2024 1,508 1 View

Experience on Data for Master Thesis on Disposition Effect in Social Trading Platforms?

Dear Forum, My master thesis investigates the disposition effect—where traders sell winning shares too early and hold onto losing shares too long—on social trading platforms (STPs) like eToro,...

08 July 2024 187 0 View

What is the most important health question facing the world today?

What global health problem does your research address? Why does it demand an urgent solution?

05 July 2024 6,163 7 View