How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Which is the best wavelength to study aerosol optical depth(AOD) related to climate reseach,which criteria must be adopted in choosing the wavelengh?

We are doing a study on impact of aerosol on precipitation. Which satellite data and which wavelength will be the best. or can some one suggest a criteria to be adopted to choose an instrument...

12 February 2021 6,324 3 View

How to compile UDF for parallel processing ?

I am trying to use a UDF for velocity input in the room from a fan. I have created a UDF but when I am trying to load the UDF it shows an error stating that it is not compiled for parallel...

03 February 2021 2,187 3 View

How do Research Gate proceed to integrate in the reference data publications and citations the recently published journals ?

How do Research Gate proceed to integrate in the reference data for publications and citations the recently published journals - for instance for the last months of 2020 and for instance Intl J...

02 February 2021 9,336 7 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

What will be the life of siRNA (liquid form) at -20 degree, if kept unopened?

Hi all, I just want to know that, if we keep the siRNA in a -20 degree freezer (as mentioned in the product detail), what will be the average life of the siRNA? Can we store for more than one...

10 January 2021 6,346 6 View

Is there a way to identify the clay mineral based on the Si:Al ratio that has been obtained through EDAX analysis?

I have come through a few literature,were the presence of particular clay mineral has been validated through the Si:Al ratio obtained from EDAX analysis. But, I would like to understand the basic...

08 January 2021 5,982 6 View

Is there any method to convert color on nitrocellulose into digits (numbers)?

This is to design a protocol in which color obtained on nitrocellulose membrane can be converted or processed through any method to digits (numbers) to compare with different state of particular...

06 January 2021 9,309 1 View

What are the inhibitors or chemicals which prevent the amplification of plant DNA? How they can be remove?

While doing amplification with Primers ( which already gave good amplification in same genus), it fails many time, eventhough the extraction has enough amont of DNA.

31 December 2020 5,649 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

Consensus sequence from ClustalX?

I am doing multiple sequence alignment to design primer for my gene. Can anyone tell me how to select the consensus sequence after alignment. I am using ClustalX for the alignment.

04 February 2021 8,872 2 View

What is the suitable buffer for this protein ?

I am trying to find a suitable buffer for storage of a His-tag purified protein. The amino acid sequence is pasted below. Can anyone help please? MMSFVSLLLV SILFPAIQAK QFTKCELPQV LKDMDGFGGI...

01 February 2021 5,216 4 View

Where can I find the receptor binding domain (amino acid sequence) of ALL pathogenic viruses?

I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?

25 January 2021 3,843 4 View

Luciferase assay for miRNA-gene: How to obtain DNA (PCR product) from the mutated primer?

To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...

19 January 2021 8,568 3 View

What Contaminates Cause Loss of Resolution in Plasmid Sanger DNA sequencing?

We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...

14 January 2021 7,346 6 View

Why this SSR primer show band like that ?

Each time this SSR primer show band like that other show one or two band . Is it polymorphic ?

11 January 2021 2,221 5 View

Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View

What is the best software to predict protein 3D structure when only amino acid sequence is available?

I want to predict the protein 3D structure by using computational tools. In this case, amino acid sequences are available only. What software would be best with maximum accuracy to predict this...

01 January 2021 444 3 View