Dear all, How feasible it is to quantify metabolites in serum using 400Mhz nmr and a broadband probe BBFO. is it possible to quantify at least 50 metabolites. Relative quantification would do. I also have AMIX software.
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View
I will be plotting multiple such shapes. Hence, I am looking for an interactive slice through option to see if there are any overlaps. Thanks,
04 February 2021 861 6 View
A new strain of the corona virus is spreading rapidly. No scientific research on symptoms and mortality yet.
22 December 2020 6,998 6 View
In gene expression studies, what would be the reliability of house keeping genes expression say in a disease/insect attacked tissue that is being subjected to studies (RT qPCR ). Will the...
14 December 2020 3,445 5 View
Need a lengthy PEEK with Zirconia based nano composite of 180 mm length. Which method is possible? Tried injection molding no proper mix. Solvent casting process is also very costly. Kindly anyone...
13 December 2020 4,329 5 View
HEK cell is Clamped at different membrane potentials (-100 mV to +90 mV; 10 mv Step with Vh -60 mV) of GluK2 receptor and recorded the current by application of 10 mM L-Glutamate. Herewith I...
07 December 2020 2,002 1 View
Mechanical impedance is a measure of how much a structure resists motion when subjected to a harmonic force. It relates forces with velocities acting on a mechanical system. At resonant...
03 December 2020 3,740 3 View
Suggestions to write a paper with good impact factor
11 November 2020 384 6 View
I am planning to conduct a cage study to determine a critical density of an univoltine casebearer moth in red clover seed crop. This requires to release first instar larvae in the cages with the...
02 November 2020 6,577 3 View
dear researchers at present we are working in fire detection projcts ,i have a plan to implement the CNN with SVM for classification method. if you hav any best or recent techniques please let me...
01 November 2020 4,142 7 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View