Dear all, How feasible it is to quantify metabolites in serum using 400Mhz nmr and a broadband probe BBFO. is it possible to quantify at least 50 metabolites. Relative quantification would do. I also have AMIX software.
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Enhancing parental satisfaction through the comprehensive debriefing of fetal anomalies using 3D models- this is my research topic. I kindly request to suggest some questions that can be asked to...
06 July 2024 8,032 0 View
BET analysis
27 June 2024 6,964 1 View
When Silica gel added in acetone, Silica gel not dissolved in acetone, but acetone form a light blue color solution , why ?
17 May 2024 4,351 0 View
We extracted PAHs from soil with acetone and then we used silica gel for clean up process , but after cleanup we found blue solution. if we used hexane, no color changed. Is this color compound...
16 May 2024 3,288 1 View
Being a researcher in the field of swarm intelligence for more than seven years, I still haven't found any research group/ institution or research lab dedicated to swarm intelligence research....
26 April 2024 4,415 2 View
I have recently published a paper in a Scopus-indexed journal. Is DOI essential? How long will it take to get DOI ? Thanks in advance
08 April 2024 4,092 3 View
Dear Networks, Seeking user-friendly software for memristor design in Neuromorphic Computing. Suggestions with links appreciated! Let's simplify the process and collaborate on efficient...
13 March 2024 5,558 1 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...
30 July 2024 906 2 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...
23 July 2024 9,421 3 View