When cells encounter stress, they will response to stress differently regarding to the types of stresses. Normally, cells will form stress granules when they are in stressful condition. I am using stress granule as a indicator to monitor cells' efficiency of stress adaptation. I first used heat shock (43 degrees,40 min) to treat the cells, and looked at stress granule number at a series of time points (0min 30 min 60 min 90 min 120 min). However, most of stress granule were gone at 30 min. In some papers, cells were treated with NaN3 30min, and it took hours to fully recover from stress. Does this mean that cells tend to recover fast from heat shock than other stress? Why do cells recover differently with different types of stress?
I guess first you precipitate some protein by heat shock. the precipitates quickly degradate or even recover after shock. in second, you kill mitochondria, which are complex cell organelles, likely degradate much slower by mitophagy. All i guess.
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