How far ethylene and leaf expansion related?

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Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...

23 January 2021 2,790 3 View

Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View

How to find porosity of a sintered ceramic compact ?

I want to know the porosity of my sintered ceramic composite ? Please help me out

21 December 2020 4,343 6 View

Precipitation of Pcl from polymeric solution ?

We working on PCL nano particles 1).Ketoprofen (10mg)+ dmso(5ml) Pcl (10mg)+ DCM(5ml) 2).Water 10 ml+ 10 mg When we mix both 1+2 after solublization (400 rpm) Why precipitation of polymer occurs?

09 December 2020 5,033 11 View

Can I include multiple outcome measures of the same construct, from the same study, in a meta-analysis?

I am carrying out a meta-analysis that looks at cognitive inhibition as an outcome. A wide range of assessment types can be used for this outcome and sometimes a study will include two or three...

15 November 2020 7,055 1 View

Can anyone suggest me an industry working in Biostimulants ?

it would be very helpful if anyone would suggest industries working in biostimulants.

25 October 2020 5,824 2 View

How can we confirm the formation of solid solution by using EDX point analysis and elemental mapping ?

I fabricated a composite say AB and both are carbides ceramics having formula AC and BC. I did EDX point analysis and observed A (45 wt %) and B (28 wt %), Carbon (26 wt %) and Oxygen (1 wt %)....

20 October 2020 7,419 7 View

Docking Approach for Combinational Drugs?

Docking studies are simple when it is a one ligand-one protein approach. However, what is the protocol for studying the combinational effect of 2 drug molecules on a particular protein, where we...

02 October 2020 8,009 22 View

How do you use the span and wagner equation of state (EOS) to calculate the mass of CO2 in a given volume of cylinder?

I am trying to use the equation of state of pure CO2 to calculate the required mass of co2 that could be contained in a given volume of cylinder.

24 September 2020 5,581 2 View

Pooling multiple continuous variables from the same study?

I am looking to meta-analyze the differential effects of physical abuse and neglect on depression in a meta-analysis. If I come across a study the reports a correlation between a continuous...

24 September 2020 4,211 3 View

What will happen (quality or quantity wise) if we transplant 'Aerobic Rice' in puddled condition?

I want to know about the consequences of aerobic rice (both quality and quantity-wise) when transplanted or sown in puddled condition?

08 February 2021 2,343 6 View

Does diseases like fungal,bacterial etc of rice plant is different from nutrient deficiency diseases?how deep learning is used except classification?

can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...

07 February 2021 470 2 View

What could be the possible reason for the drastic variation in Qmax value for same adsorbent onto two different cationic dyes itself?

The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?

30 January 2021 1,420 3 View

What are the new researchable area under Rice crop under Agronomy related to moisture stress and nanotechnology ?

I had already worked on the moisture stress and lot of works being done in rice regarding stress or drought. I want to know some very recent researchable areas in rice crop regarding moisture...

26 January 2021 5,518 7 View

Can anyone help me to separate my phenolic compound in TLC with any new solvent system ?

My Compound is Leaf powdered Methanolic extract which is showing good phenolic compounds upon dipping in FeCl3. -My problem is phenolic compounds are not eluting with any common solvent system.

22 January 2021 4,693 7 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Suggest best performance evaluation metrics for image segmentation?

Good morning everyone. As a part of my research work, I designed a network that extracts the leaf region from the real field images. when I search about performance evaluation metrics for the...

10 January 2021 9,581 30 View

Easy and effective method to visualize fungal mycelium inside leaf tissue?

Is there any easy and efficient method to visualize the fungal mycelium inside infected leaf tissue using light microscoopy? Please share protocols which can be performed using limited resources...

03 January 2021 4,217 2 View

Is it possible to separate the momilactone molecule by GPC device?

The momilactone molecule is present in rice and has an allochemical effect on weeds.

01 January 2021 3,367 3 View

How can I use amylase enzyme to digest molluscan tissue before DNA extration?

Hi, I have a problem with a hight contet of mucopolysaccharides in some molluscan samples. I tried different DNA extration procolols but I can't already remove all the molecules that inhibit PCR...

01 January 2021 8,900 3 View

Anoop Kumar Srivastava

Ethylene is generally known for multiple functions , right from preevaluation stage to tolerance against diverse biotic and abiotic stress. But in rice, under waterlogged conditions , ethylene plays a harmonal role in leaf growth, mostly the positive effect on leaf expansion...

Nirmala S.V.S.G

The role of ethylene in the leaf growth and development has been confirmed ..... In Arabidopsis, through the growth comparison of ethylene-related mutants, .... whereas stimulation in long-day (LD) plants under non-inductive