Hello Fatima Daigan

The in vitro measurement of haemoglobin derivates by multiwavelength oximeters is influenced by hyperbilirubinaemia. Severe jaundice is one of these mechanisms with some clinical interest. In the literature it is pointed out that a high bilirubin concentration may falsify oximetric measurements and is often accompanied by elevated COHb levels. This is caused by an interference between the light absorption spectra of the haemoglobin derivates and of bilirubin and by the increasing development of endogenous CO in the haem metabolism during severe jaundice.

You could correct for the presence of interfering substances by the use of an Allen correction, or by derivative spectrophotometry, or by polychromatic analysis.

I have attached some references which could refer for more information.

1. Harboe M. A method for determination of hemoglobin in plasma by near ultraviolet spectrophotometry. Scand J Clin Lab Invest 11, 66-70 (1959).

2. Caraway WT. Mathematics in clinical chemistry. In Clinical Biochemistry: Contemporary Theories and Techniques, 1, HE Spiegel, Ed., Academic Press, New York, NY, 1981, pp 93-94.

3. Blakney GB, Dinwoodie AJ. A spectrophotometric scanning technique for the rapid determination of plasma hemoglobin. Gun Biochem 8, 96-102 (1975).

4. Amazon K, Soloni F, Rywlin AM. Separation of bilirubin from hemoglobin by recording derivative spectrophotometry. Am J Gun Pathot 75, 519-523 (1981).

5. Dennis A. Noe, Victor Weedn, William R. Bell. Direct Spectrophotometry of Serum Hemoglobin: An Allen Correction Compared with a Three-Wavelength Polychromatic Analysis. CLIN. CHEM. 30/5, 627-630 (1984).

Best.