How does Illumina sequencing work?

More Anurup Mohanty's questions See All

What is the reason for arising of a negative peak during a sample run in an HPLC system?

What could be the reason behind a negative peak mostly arising during the initial stage of sample run (RT 1-10).

15 February 2021 4,231 4 View

How do I scale up a column for preparative isolation/purification from analytical conditions in HPLC?

What protocol should I follow to determine optimum separation conditions using an analytical column in an HPLC system?

03 February 2021 7,288 3 View

How to calculate the polysaccharide content of a curcuma species through HPLC method?

what all standards are needed to quantify and analyze the composition with a proper method fie for the mobile phase to run the sample ?

31 January 2021 7,704 4 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

How can I check the chemical composition of an essential oil using HPLC analysis?

Injected an oil having a concentration of 832mg/ml and found a flat top peak and was not able to quantify. How to resolve this problem?

20 January 2021 8,461 3 View

How to simulate desiccation in molecular dynamics?

I'm trying to study the effects of desiccation on some proteins, could someone tell what solvent or what simulation conditions I should set up to simulate a desiccation environment on GROMACS.

05 January 2021 7,033 3 View

What is diffrence between coupling buffer and immobilization buffer in SPR experiment?

i am performing SPR experiment and i am referring some journal and websites they stated regarding coupling buffer and immobilization buffer so ihave confusion about these buffer.

06 December 2020 470 3 View

What is the scope of doing PhD in Virology in the UK?

With the great interest in Virology in the world due to the COVID-19 pandemic and its related vaccine development is doing a Phd in Virology from a good institute in UK the right thing to do?

26 November 2020 3,681 3 View

Is it a feasible way to model CO2 emissions Mathematically?

I am working on the Environmental and Social indicators. So the easiest way is to get the solution is to go for any LCA tool;But my doubt is ,is it possible to mathematically model the Emissions...

17 November 2020 8,492 3 View

How to fix this gromacs error?

Residue 6 named GLU of a molecule in the input file was mapped to an entry in the topology database, but the atom CG used in that entry is not found in the input file. Perhaps your atom and/or...

16 November 2020 1,110 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Abhijeet Singh

Your gaps and concepts would/could have already been solved if you have searched the exact heading of your question in your browser. Also, there are hundreds of discussion are available even here on researchgate.

Aissa Saidi

https://books.google.co.ma/books?id=TGqQDwAAQBAJ&printsec=frontcover&dq=illumina+sequencing&hl=en&sa=X&ved=2ahUKEwiWqMKNjtz1AhXM5-AKHf_jDYgQ6AF6BAgIEAI#v=onepage&q=illumina%20sequencing&f=false

David Novak

I recently came across this post https://kscbioinformatics.wordpress.com/2017/02/13/illumina-sequencing-for-dummies-samples-are-sequenced/ and it does a decent job explaining it I think.

Not implying anything :)