How does Hydrogen peroxide bleach the fluorescence signal (in IHC)?

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P. Pardha-Saradhi

Hydrogen peroxide will bleach fluorescence signal by oxidizing the molecules/complex responsible for emitting fluorescence.

Steingrimur Stefansson

Hi Kübra, are you aiming to bleach background autofluorescence due to aldehyde fixation of brain tissues?

You could try sodium borohydride (NBH4), which reduces fluorescencent carbonyl compounds caused by aldehyde fixation.

http://www.sciencedirect.com/science/article/pii/S0165027098000661