Dear colleagues,

I am using BIOLOG microstation and AN plates to do the substrate consumption fingerprint of a strain in different culture media. The machine gives you pink colored wells as a positive result. I am trying to come up with the rule it follows to assign this positives according to the absorbance. I have tried assigning positives to the outliers, values above Q3 or Q2 but it did not match Biolog positives assignation so far.

Any suggestions? I want to use a binary matrix to cluster strains according to if they consume the source or not

Thank you in advance,