For example, on the Born aggregometer, we see 2 waves of platelet aggregation upon addition of agonist ADP - the primary wave and secondary wave.
In this paper, I read the following: "At critical concentrations, weak agonists such as epinephrine and ADP induce a biphasic response. The primary wave of aggregation reflects response to the addition of exogenous agonist. When normal platelets are activated, they secrete an endogenous pool of agonists. The secondary wave represents aggregation in response to this endogenous pool".
Does this statement imply that we do not see the primary wave of aggregation in vivo? As I thought we do see the primary wave of aggregation in any scenario since it is the activation of the P2Y1 pathway, mobilisation of calcium from intracellular stores and platelet shape change.
Is it possible to get the secondary wave of aggregation without the primary wave? In what scenario?
https://ashpublications.org/thehematologist/article/doi/10.1182/hem.V11.2.2555/462563/Light-Transmission-Aggregometry
Dear Rh Rj
I had worked with 20uM ADP and at this concentration, we get only the secondary wave. To the best of my knowledge the primary wave is observable at low concentration mimicking in vivo conditions
Rh Rj
Yes, with 20uM ADP we only observed a decrease in absorbance/optical density. To the best of my knowledge optical density is the absorbance of light passing through a transparent medium. Besides, I want to add that we measured it in 96-well transparent plate instead of a cuvette. I hope it may be helpful to you.- Badges
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