How does a molecule selectively block virus-triggered IRF3 phosphorylation without disrupting upstream innate immune signaling?

13 July 2024 1 9K Report

I observed a molecule that strongly inhibits virus-induced IRF3 activation while leaving upstream innate immune signals like TBK1 phosphorylation and MAVS levels unaffected. How might this molecule selectively inhibit IRF3 phosphorylation? What experiments could validate mechanistic basis of this observed phenotype? Suggest me please!

Kul Raj Rai

Finally solved: This appears to occur through the interaction of that molecule with MAVS via its DLAIS motif!!!!!!!!

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