Kul Raj Rai

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Questions related from Kul Raj Rai

How does a molecule selectively block virus-triggered IRF3 phosphorylation without disrupting upstream innate immune signaling?

I observed a molecule that strongly inhibits virus-induced IRF3 activation while leaving upstream innate immune signals like TBK1 phosphorylation and MAVS levels unaffected. How might this...

13 July 2024 9,495 1 View

Why does molecular weight (MW) of a protein expressed in prokaryotic system differ from its theoretically expected MW?

We cloned a viral gene into pET28a expression vector and expressed in E. coli. Then, expressed protein was examined by WB and other methods. However, unexpectedly, molecular weight (MW~35KD) of...

24 June 2021 9,980 3 View