Hello.
I’m working on a viral titration assay validation plan (plaque forming assay).
From my literature search, I understand that accuracy of this type of assay is reasonable if titres within 0.5log10 of the nominal titre are achieved.
So, if the nominal titre of a sample is e.g. 1 x 10^8 PFU/ml, what is +0.5log10 of this, and what is -0.5log 10 of this i.e. the acceptable range for observed titres?
I’ve done so much literature reading, but still can’t work it out. Thanks in advance for answers.
Half a log10 is approximately 3-fold (actually the square root of 10, which is just above 3). So +/- 0.5log10 translates to ~3-fold above or below, hence about 0.3 x108 to 3 x 108 PFU/ml.
0.5log10 is ~3-fold. So your titer range would come in 3-fold above or below your target pfu. General rule of thumb in this type of assay is that your accuracy will generally be +/- one dilution unit. So if you are doing 2-fold dilutions of your virus you will be accurate within a 4-fold range. That is about as good as a plaque assay gets.
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays