Do anyone know better method to increase efficiency of transient transfection of k48R mutant ubiquitin plasmid?

I am trying to transfect brain endothelial cells using K48R mutant ubiquitin plasmid using Lipofectamin 2000 as well as lipofectamin 3000. But transfection efficiency is just 5%. Can some one...

31 December 2017 2,462 0 View

Have anybody used far red fluorescence dye for staining bacteria for live microscopic imaging?

I am searching for a fluorescence far red dye to stain bacteria for live Microscopic Imaging. PSVUE is one of NIR (Near Infrared Dye) which stains anionic lipids but it is not compatible with PBS...

09 October 2016 1,850 0 View

Good Ubiquitin ligase inhibitor to block ubiquitination in HBMECs In Vitro

HI. I am trying to inhibit ubiquitination in Human Brain Endothelial Cells In Vitro using PYR-41 ubiquitin ligase inhibitor. But it is not inhibiting ubiquitination as lower concentration and at...

07 August 2016 8,242 2 View

IS there a staining for adherent and tight junction protein in fixed cells?

04 May 2015 8,288 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View